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  Use our Product Finder to view and compare products that meet your specific requirements.
- Imaging & Microscopy
  Automated Cell Imagers
  
  Lionheart FX Automated Microscope
  Lionheart LX Automated Microscope
  Cell Imaging Multi-Mode Readers
  
  Cytation 7 Cell Imaging Multi-Mode Reader
  Cytation 5 Cell Imaging Multi-Mode Reader
  Cytation 1 Cell Imaging Multi-Mode Reader
  Multi-Plate Live Cell Imaging System
  
  BioSpa Live Cell Analysis System
  Peripherals
  
  Cell Count & Viability Starter Kit
  Scratch Assay Starter Kit
  Gas Controller
  Dual Reagent Injector Module
  Product
  Comparison Charts
  
  Imaging & Microscopy
  
  Gen5 Edition Comparison
- Detection
  Multi-Mode Microplate Readers
  
  Synergy Neo2 Hybrid Multi-Mode Reader
  Cytation Hybrid Multi-Mode Reader
  Synergy H1 Hybrid Multi-Mode Reader
  Synergy HTX Multi-Mode Reader
  Synergy LX Multi-Mode Reader
  Multi-Plate Absorbance Reader
  
  LogPhase 600 Microbiology Reader
  Absorbance Microplate Readers
  
  Epoch 2 Microplate Spectrophotometer
  Epoch Microplate Spectrophotometer
  ELx808 Absorbance Reader
  800 TS Absorbance Reader
  Peripherals
  
  Gas Controller
  Take3 Plate
  Dual Reagent Injector Module
  Product Comparison Charts
  
  Multi-Mode Readers
  
  Absorbance Readers
  
  Microplate Spectrophotometers
  
  Gen5 Edition Comparison
- Liquid Handling
  Multi-Mode Washer Dispensers
  
  EL406 Washer Dispenser
  MultiFlo FX Multi-Mode Dispenser
  Microplate Washers
  
  405 TS Washer
  405 LS Washer
  ELx405 Select Deep Well Washer
  50 TS Washer
  Microplate Dispensers
  
  MultiFlo FX Multi-Mode Dispenser
  MicroFill Dispenser
  Product Comparison Charts
  
  Microplate Washers
  
  Microplate Dispensers
- Software
  Software
  
  Gen5 Software Features for Imaging & Microscopy
  Gen5 Software Features for Detection
  Liquid Handling Control Software
  Software Apps
  
  Scratch Assay App
  Quantitative Assay Apps
  Cell Count & Viability App
  LogPhase 600 App
  Product Comparison Charts
  
  Gen5 Edition Comparison
- Robotics
  Robotics
  
  BioSpa 8 Automated Incubator
  BioStack Microplate Stacker
  BenchCel Microplate Handler
  Product Comparison Charts
  
  BioStack Configuration Comparison
- Buy Cell Culture Microplates
  Sourcing your microplates shouldn't be an added task in preparing your cell-based workflows. Agilent now offers cell culture and imaging microplates so you can complete your workflows seamlessly.
  
  Click here to request your free sample.
- Technologies
  Multi-Mode Detection Technologies
  
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  TR-FRET
  Imager & Microplate Reader Technologies
  
  4-Zone Incubation and Condensation Control
  Augmented Microscopy
  Hybrid Technology
  Laser Autofocus
  Peltier Cooling Module
  Variable Bandwidth Monochromators
  Liquid Handling Technologies
  
  Automated, Gentle Media Exchange
  Dual-Action Manifold
  Parallel Dispense
  Random Access Dispense
  Ultrasonic Advantage
  Verify Technology
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Peripherals
- Peripherals
  BioTek’s peripheral products help to expand your BioTek instrument applications and improve workflow efficiency. From gas controllers to micro-volume plates, we have the peripherals to help advance your research.
- Accessories
  To complete or enhance your BioTek imager, microplate reader, liquid handling or robotics system, we offer a wide range of accessories, from tubing and bottles to imaging filter cubes. Search our Accessory database for Accessories compatible with your instrument. Search by Keyword, Part #, Product or Accessory Type.
- Application Starter Kits
  BioTek’s Application Starter kits contain everything you’ll need to implement the latest assays for a particular application, quickly and with minimal training. Kits contain hardware, software, consumables and reagents all in a convenient kit.
Applications
- Cell Culture QC
  
  Automated imaging tools can provide valuable information for improving routine cell culturing techniques and increasing the effectiveness and reproducibility of downstream cell-based assays. Conventional methods for evaluating cell culturing techniques and assay design consist of manual inspection of a small subset of the cell population at random locations and time points.
- Cell Health and Viability
  
  Cell health and viability measurements provide essential insight into a broad range of biological processes and treatment responses. Diverse assay formats and reagents have been developed that measure specific aspects of cell viability corresponding to particular cellular response pathways and mechanisms of injury.
- Cell Metabolism
  
  Cellular metabolism comprises a number of biochemical reactions that occur in concert within the cells of living organisms. These orchestrated reactions, better described as metabolic pathways allow organisms to function and respond to environmental changes. Agilent cell metabolism assays detect discrete changes in cell bioenergetics in real time, providing a window into the critical functions that provide ATP, the energy that cells need for activation, signaling, proliferation, and biosynthesis.
- Cell Migration and Invasion
  
  Cell migration and invasion are live cell kinetic assays useful in oncology research. The principle difference between migration and invasion is that migration refers to normal cell movement; whereas invasion describes cells actively invading surrounding tissue. It can do this under the influence of a chemoattractant gradient and secretion of proteases. There are numerous methods for performing these assays including scratch assays performed on a monolayer of cells adhered to plasticware (microplates or culture inserts) or using 3D cell culture models.
- Developmental Biology
  A macro trend in drug discovery is the use of complex models of disease early in preclinical studies. An approach following this viewpoint is the use of whole organism models involving living invertebrates and vertebrates such as nematodes and zebrafish conducive to drug screening methods.
- Histology and Slide Scanning
  Histology is typically performed on fixed tissue samples using upright microscopes equipped with oculars. Cytation™ and Lionheart™ FX provide automated digital imaging of these samples using color brightfield microscopy. Low to high magnifications from 1.25x to 60x (100x with Lionheart FX) can be combined with Cytation Gen5™ image processing features such as image stitching to increase field of view.
- Immunology
  The immune system relies on diverse mechanisms working in concert to defend the host from infection and to identify and remove aberrant or damaged cells. Dysregulation of the immune system can lead to a variety of disease conditions, including autoimmune disorders, allergies, and cancer. Additionally, the immune system is a critical consideration for vaccine development and cell, tissue and organ transplants.
- Immuno-Oncology
  While harnessing the patient’s immune system to fight cancer is not a new concept, there have been significant strides in the past decade to more effectively direct and activate components of the immune system and counter the measures certain tumors adopt to shield itself from immune attack. These include immunotherapeutic drugs based on directing immune cells to tumors based on specific antigens they present, PD-1 checkpoint inhibition and the ex vivo manipulation of the patient’s own immune cells to better drive an immune response to cancer.
- Microbiology
  Since the advent of a lens system that enabled the visualization of bacteria and protists in 1671, the microscope remains a ubiquitous tool for the scientific study of live and fixed microorganisms. Investigating the structure, function, and typing of the highly diverse array of microorganisms spans heterogeneous applications including, but not limited to, the elucidation, diagnosis, and treatment of human and plant disease, food and water safety testing, vaccine development, investigation and production of industrial lipases, protein transfection to mammalian cells, environmental health and impact appraisal, horticultural and agricultural soil assessment, and exploration of alternative fuel sources.
- Molecular Biology and Biochemical
  Biochemical assays run the gamut from routine ELISAs to micro-volume nucleic acid and protein quantification. Endpoint and kinetic, single- and multi-wavelength assays are essential experimental tools. BioTek’s multi-mode readers have the flexibility to accommodate a very broad range of applications with superior performance.
- Rapid Cell Signaling
  G-protein coupled, seven-transmembrane (7-TM) receptors (GPCRs) represent large, diverse gene families found in fungi, plants and animals. The GPCR family of receptors is involved primarily with internalizing extracellular environmental signals via interaction with intracellular G-proteins. GPCR ligands and modulators range from peptide and non-peptide molecules to chemokines, nucleosides, growth factors and light.
- Stem Cell Research
  Research into the reprogramming of somatic cells into pluripotent stem cells received the Nobel Prize in 2012. The long term importance of this research resides in the use of stem cells for regenerative therapies; for the short term, however these cells represent a potential unlimited source of highly physiologically relevant cells, akin to human primary cells, that can be used as drug discovery reagents.
- Virology
  Research into the diverse aspects of virology are made possible by a broad range of applications and assay formats, from relatively simple methods for viral detection to those designed to elucidate complex immune responses and pathological processes. BioTek instruments provide versatile platforms for conducting viral research, with optimized imaging- and plate reader-based solutions, as well as liquid handling and automation tools for increased assay throughput and reproducibility.
Resources
- Application Notes
  
  BioTek’s application notes present the latest-breaking scientific content covering the vast range of applications across our entire product line. Browse applications by topic, detection and product or use the simple keyword search to find the application notes that will facilitate your research.
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- Visual Abstracts
  BioTek’s Visual Abstracts are brief, animated presentations that describe the workflow of a single specific application. Imaging & microscopy-focused Visual Abstracts explain the Augmented Microscopy workflow for a wide range of assays, with key capture>process>analyze>publish concepts presented. Other Visual Abstracts describe the utility of BioTek’s liquid handling and multi-mode instruments in a variety of applications. Check back often for our latest additions to the Visual Abstract library!
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About Us
- News
  Synergy H1’s Continuously Variable Bandwidth Monochromators Optimize Assay Performance
  July 2, 2020 - BioTek Instruments announces the availability of variable bandwidth monochromators on their modular and versatile Synergy? H1 Hybrid Multi-Mode Reader. This allows researchers to achieve even greater levels of assay sensitivity and specificity compared to fixed bandwidth systems. Read more
  
  LogPhase 600 Microbiology Reader Measures Microbial Growth Curves in Multiple Microplates
  June 9, 2020 - BioTek unveils a unique class of microplate readers with the new LogPhase 600 Microbiology Reader. The LogPhase 600 is the only four-plate microplate reader purpose-built to perform microbial growth curve analysis. Multiple plate measurements increase throughput and reduce the need for multiple costly single-plate absorbance readers that take up valuable bench space. At the same time, highly consistent environmental conditions support high quality absorbance results without growth curve artifacts, even in bacterial and yeast assays with extended incubation periods. Read more
  
  Agilent’s BenchCel Microplate Handler Now Available through BioTek for Full Workflow Automation
  May 28, 2020 - BioTek announces that Agilent’s BenchCel Microplate Handler is now added to its portfolio of walkaway automated solutions. The modular BenchCel Microplate Handler is a high-speed robot with an open, flexible and scalable format to bring efficiency to diverse applications such as ELISA, endpoint add-and-read assays and cell fix-stain-image processes. Read more
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  April 9 - 14, 2021
  
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  Washington D.C.
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Home Products Detection Synergy H1 Hybrid Multi-Mode Reader

Synergy H1 Hybrid Multi-Mode Reader

Download Literature Get more info Request Quote

Synergy™ H1 is a configurable multi-mode microplate reader, with monochromator-based optics for flexibility, filter-based optics for sensitivity, or both…BioTek’s patented Hybrid Technology™ offers applications versatility and excellent performance in a modular platform to expand as your laboratory’s needs change. Synergy H1 now offers continuously variable bandwidth monochromators for fluorescence excitation and emission wavelength selection; the fluorescence bandwidth can be set between 9 nm and 50 nm, in 1 nm increments, allowing users to fully optimize reader settings to drive the best assay performance compared to fixed bandwidth systems.

Sourcing your microplates shouldn't be an added task in preparing your cell-based workflows. Agilent now offers cell culture and imaging microplates so you can complete your workflows seamlessly. Click here to request your free sample.

What's New

A Seamless Automated Workflow for Plate Reader Assay Sample Prep

The Agilent Bravo Liquid Handling system works hand-in-hand with BioTek Synergy Multi-Mode Plate Readers to deliver automated sample prep for standardizing and optimizing plate reader assays.

LEARN MORE

Cell Metabolism: MitoXpress and pH-Xtra Assays

Download our Application Note to discover how combining MitoXpress and pH-Xtra reagents with BioTek plate readers provide sensitive detection methods, including advanced lifetime (µs) time-resolved fluorescence (TRF), for getting the most meaningful data out of your cell metabolism assays.

DOWNLOAD

applications

With quadruple variable bandwidth monochromators and optional gas control and reagent injection, Synergy H1 can be used for a wide range of applications in fluorescence, luminescence, UV-Vis and more.

Cell based assays
Biomarker Assays
Micro-volume analysis of nucleic acid and protein
Kinetic assays
Micro-volume nucleic acid and protein quantification with the Take3 Micro-Volume Plate

Effect of increasing levels of ethanol on yeast growth

HTRF® ratios for AMD3011 and SDF1-alpha

Micro-volume (2 µL) quantification of up to 48 samples with Take3 Plate

These are just a few examples of the many important applications for Synergy H1. Visit our Applications page to see more.

BioTek Field Applications Scientists (FAS) and Product Specialists provide unparalleled scientific support, assisting with experimental planning and assay optimization on BioTek’s instrumentation and software. Learn how they can help move your science forward… faster!

Features

Hybrid plate reader: Flexibility and performance

With its patented combination of monochromator and filter optics, Synergy H1 is an advanced plate reader
that delivers both the flexibility and performance you need for any microplate assay in your lab.

Monochromator: variable bandwidth, absorbance, fluorescence, luminescence
Filters: fluorescence polarization, time-resolved fluorescence

Upgradable to meet future application needs

Synergy H1’s modular design allows you to start with what you need now, and add detection modes, gas control and dual reagent injectors as your laboratory’s workflows evolve.

Variable bandwidth for sensitivity and specificity

Advanced microscopy: Unlimited possibilities

Synergy H1 offers quad monochromator optics with variable bandwidth. The bandwidth can be set between 9 nm and 50 nm, in 1 nm increments. Large bandwidths (1) provide increased sensitivity and lower limits of detection. Small bandwidths (2) provide increased specificity when multiple signals are present, reducing crosstalk and enhancing assay performance.

Automated z-focus: best performance with all plate types Micro-volume analysis with Take3 plate

(1) Without automated z-focus available, performance at low volumes is affected. (2) With automated z-focus, reading height is precisely adjusted for best performance in all plate types and all volumes.

Extended dynamic range

Synergy H1 offers an extended dynamic range, which allows detection of signals across a 7 log measurement range. Other systems can measure only small portions of the dynamic range of Synergy H1 using preset gains – this can cause reduced sensitivity on the low end or saturated signals on the high end of the assay signal range.

Environmental controls for cell-based assays

Temperature control to 45 °C, CO₂/O₂ control and condensation control and shaking create the ideal environment for live cell assay workflows. A consistent environment leads to consistent data for long-term kinetic assays.

Dual syringe injectors with specialized tips

1) The robust precise dual syringe design eliminates the need for regular tubing replacement required by some peristaltic injector designs. Synergy H1 offers two tip types: (2) the straight tips enable vigorous mixing for rapid inject/read assays, and (3) the angled tip option won’t disturb cell layers for applications such as calcium kinetics.

Micro-volume analysis with Take3 plate

Enable micro-volume analysis with the Synergy H1, using the Take3 Plate. Measure up to 16 or 48
samples in one run and save a lot of time, compared to single-sample devices. Gen5 has customizable
protocols for ssDNA, dsDNA, RNA and protein quantification in 2 µL.

Product Reviews

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Customer Spotlight

Biocontrol of Invertebrate Pests Using Natural Agents: Investigating Host-Pathogen Interactions with the BioTek Synergy H1

24-June-15

Biocontrol agents provide a safer alternative to chemical pesticides and insecticides for the agricultural and forestry industries, as well as human health. In this video, Dr Bethany Greenfield, Post-Doctoral Research Assistant in the Biocontrol and Natural Products (BANP) Group at Swansea University, describes her research into the use of Metarhizium anisopliae for the control of mosquitoes. Hear how the BioTek Synergy H1 enables the group to efficiently perform a range of analyses, from protein quantification to measuring insect stress response.

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