Synergy H1 Hybrid Multi-Mode Reader

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Synergy™ H1 is a configurable multi-mode microplate reader, with monochromator-based optics for flexibility, filter-based optics for sensitivity, or both…BioTek’s patented Hybrid Technology™ offers applications versatility and excellent performance in a modular platform to expand as your laboratory’s needs change. Synergy H1 now offers continuously variable bandwidth monochromators for fluorescence excitation and emission wavelength selection; the fluorescence bandwidth can be set between 9 nm and 50 nm, in 1 nm increments, allowing users to fully optimize reader settings to drive the best assay performance compared to fixed bandwidth systems.

What's New

Purchase a Synergy or Cytation Reader for use with Agilent MitoXpress or pH-Xtra Assays and receive a free filter cube!

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Download our Application Note to discover how combining MitoXpress and pH-Xtra reagents with BioTek plate readers provide sensitive detection methods, including advanced lifetime (µs) time-resolved fluorescence (TRF), for getting the most meaningful data out of your cell metabolism assays.

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applications

With quadruple variable bandwidth monochromators and optional gas control and reagent injection, Synergy H1 can be used for a wide range of applications in fluorescence, luminescence, UV-Vis and more.

Cell based assays
Biomarker Assays
Micro-volume analysis of nucleic acid and protein
Kinetic assays
Micro-volume nucleic acid and protein quantification with the Take3 Micro-Volume Plate

Effect of increasing levels of ethanol on yeast growth

HTRF® ratios for AMD3011 and SDF1-alpha

Micro-volume (2 µL) quantification of up to 48 samples with Take3 Plate

These are just a few examples of the many important applications for Synergy H1. Visit our Applications page to see more.

BioTek Field Applications Scientists (FAS) and Product Specialists provide unparalleled scientific support, assisting with experimental planning and assay optimization on BioTek’s instrumentation and software. Learn how they can help move your science forward… faster!

Features

Hybrid plate reader: Flexibility and performance

With its patented combination of monochromator and filter optics, Synergy H1 is an advanced plate reader
that delivers both the flexibility and performance you need for any microplate assay in your lab.

Monochromator: variable bandwidth, absorbance, fluorescence, luminescence
Filters: fluorescence polarization, time-resolved fluorescence

Upgradable to meet future application needs

Synergy H1’s modular design allows you to start with what you need now, and add detection modes, gas control and dual reagent injectors as your laboratory’s workflows evolve.

Variable bandwidth for sensitivity and specificity

Advanced microscopy: Unlimited possibilities

Synergy H1 offers quad monochromator optics with variable bandwidth. The bandwidth can be set between 9 nm and 50 nm, in 1 nm increments. Large bandwidths (1) provide increased sensitivity and lower limits of detection. Small bandwidths (2) provide increased specificity when multiple signals are present, reducing crosstalk and enhancing assay performance.

Automated z-focus: best performance with all plate types Micro-volume analysis with Take3 plate

(1) Without automated z-focus available, performance at low volumes is affected. (2) With automated z-focus, reading height is precisely adjusted for best performance in all plate types and all volumes.

Extended dynamic range

Synergy H1 offers an extended dynamic range, which allows detection of signals across a 7 log measurement range. Other systems can measure only small portions of the dynamic range of Synergy H1 using preset gains – this can cause reduced sensitivity on the low end or saturated signals on the high end of the assay signal range.

Environmental controls for cell-based assays

Temperature control to 45 °C, CO₂/O₂ control and condensation control and shaking create the ideal environment for live cell assay workflows. A consistent environment leads to consistent data for long-term kinetic assays.

Dual syringe injectors with specialized tips

1) The robust precise dual syringe design eliminates the need for regular tubing replacement required by some peristaltic injector designs. Synergy H1 offers two tip types: (2) the straight tips enable vigorous mixing for rapid inject/read assays, and (3) the angled tip option won’t disturb cell layers for applications such as calcium kinetics.

Micro-volume analysis with Take3 plate

Enable micro-volume analysis with the Synergy H1, using the Take3 Plate. Measure up to 16 or 48
samples in one run and save a lot of time, compared to single-sample devices. Gen5 has customizable
protocols for ssDNA, dsDNA, RNA and protein quantification in 2 µL.

Product Reviews

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Customer Spotlight

Biocontrol of Invertebrate Pests Using Natural Agents: Investigating Host-Pathogen Interactions with the BioTek Synergy H1

24-June-15

Biocontrol agents provide a safer alternative to chemical pesticides and insecticides for the agricultural and forestry industries, as well as human health. In this video, Dr Bethany Greenfield, Post-Doctoral Research Assistant in the Biocontrol and Natural Products (BANP) Group at Swansea University, describes her research into the use of Metarhizium anisopliae for the control of mosquitoes. Hear how the BioTek Synergy H1 enables the group to efficiently perform a range of analyses, from protein quantification to measuring insect stress response.

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