Cytation Hybrid Multi-Mode Reader
Technical Details
General
| Detection modes |
UV-Vis absorbance Fluorescence intensity Luminescence Fluorescence polarization (Cytation 5/1) Time-resolved fluorescence (Cytation 5/1) Alpha (Cytation 5) |
| Read methods | Endpoint, kinetic, spectral scanning, well area scanning |
| Microplate types |
Monochromator: 6- to 384-well plates Filters: 6- to 1536-well plates Imaging: 6- to 1536-well plates |
| Other labware supported |
Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer) Take3 Micro-Volume Plates |
| Temperature control |
4-Zone incubation to 45 °C (Cytation 7/1) with Condensation Control™ 4-Zone incubation to 65 °C (Cytation 5) with Condensation Control |
| Cooling | Optional Peltier Cooling Module maintains internal temperature with < 1 °C rise over ambient. Provides internal cooling after incubated processes. |
| Shaking | Linear, orbital, double orbital |
| Software |
Gen5™ Microplate Reader and Imager Software Gen5 Secure for 21 CFR Part 11 compliance (option) |
| Automation |
BioStack and 3rd party automation compatible BioSpa 8 Automated Incubator compatible |
| CO2 and O2 control (option) |
Range: 0 - 20% (CO2); 1 - 19% (O2) Models for both CO2 and O2 or CO2 only are available |
Absorbance
| Light source | Xenon flash |
| Detector | photodiode |
| Wavelength selection | monochromator |
| Wavelength range |
230 - 999 nm, 1 nm increments (Cytation 7/5) 200 - 999 nm, 1 nm increments (Cytation 1) |
| Monochromator bandwidth |
4 nm (230 - 285 nm), 8 nm (>285 nm) (Cytation 7/5) 2.4 nm (Cytation 1) |
| Dynamic range | 0 - 4.0 OD |
| Resolution | 0.0001 OD |
| Pathlength correction | yes |
| Monochromator wavelength accuracy | +2 nm |
| Monochromator wavelength repeatability | +0.2 nm |
| OD accuracy |
<1% at 2.0 OD <3% at 3.0 OD <1% at 3.0 OD (Cytation 7) |
| OD linearity | <1% from 0 to 3.0 OD |
| OD repeatability | <0.5% at 2.0 OD |
| Stray light | 0.03% at 230 nm |
| Reading speed (kinetic) |
96 wells: 11 seconds 384 wells: 22 seconds |
Fluorescence Intensity
| Light source | Xenon flash |
| Detector | PMT |
| Wavelength selection |
Quad monochromators (top/bottom) (Cytation 7/5) Filters / dichroic mirrors |
| Wavelength range |
Monochromators: 250 - 700 nm (900 nm option) (Cytation 7/5) Filters: 200 - 700 nm (850 nm option) |
| Monochromator bandwidth | Variable; from 9 to 50 nm, in 1 nm increments (Cytation 7/5) |
| Dynamic range | 7 decades |
| Sensitivity |
Filters: Fluorescein 0.25 pM (0.025 fmol/well, 384-well plate) Quad Monochromator (Cytation 7/5): Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) - top Fluorescein 4 pM (0.4 fmol/well, 384-well plate) - bottom |
| Reading speed (kinetic) |
96 wells: 11 seconds 384 wells: 22 seconds |
Luminescence
| Wavelength range | 300 - 700 nm |
| Dynamic range | >6 decades |
| Sensitivity |
Monos: 20 amol ATP (flash) (Cytation 7/5) Filters: 10 amol ATP (flash), 100 amol (glow) |
Fluorescence Polarization
| Light source | Xenon flash |
| Detector | PMT |
| Wavelength selection | Filters |
| Wavelength range | 280 - 700 nm (850 nm option) |
| Sensitivity | 1.2 mP standard deviation at 1 nM fluorescein |
Time-Resolved Fluorescence
| Light source | Xenon flash |
| Detector | PMT |
| Wavelength selection |
Quad monochromators (secondary mode) (Cytation 5) Filters (top) |
| Wavelength range | Filters: 200 - 700 nm (850 nm option) |
| Sensitivity |
Filters: Europium 40 fM (4 amol/well, 384-well plate) Monos: Europium 1200 fM (120 amol/well, 384-well plate) (Cytation 5) |
Alpha Detection
| Light source | 100 mW 680 nm laser (Cytation 5) |
| Detector | PMT (Cytation 5) |
| Wavelength selection | Filters (top) (Cytation 5) |
| Sensitivity | 100 amol LCK peptide (384-well low volume plate) (Cytation 5) |
Reagent Dispensers
| Supported detection modes | All modes |
| Number | 2 syringe pumps |
| Supported labware | 6- to 384-well microplates, Petri dishes |
| Dead volume | 1.1 mL, 100 µL with back flush |
| Dispense volume | 5 - 1000 µL in 1 µL increments |
| Dispense accuracy | +1 µL or 2% |
| Dispense precision | <2% at 50 - 200 µL |
Physical Characteristics
| Power | 150 Watts to 250 Watts, configuration dependent |
| Dimensions |
20.2" D x 16.4" W x 17.5" H (51.4 cm D x 41.6 cm W x 44.5 cm H) |
| Weight | 65 lbs to 80 lb (29 kg to 36.3 kg), configuration dependent |
Regulatory
| Regulatory | CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available. |