Resources
265 Results:
Application Notes
3D Cell Culture (25)
In-vitro cell-based assays that utilize a 3D structure of aggregated cells using hydrogels, scaffolds or spheroids to provide more physiologically relevant data.
- Automated Image Screening of Zebrafish Embryos Exposed to Developmental Toxins (January 4, 2019)
- Automated Media Exchange for Spheroid Cultures Using a Novel MultiFlo? FX Accessory (July 31, 2018)
- 3D Spheroid-Based Tumor Invasion Assay (July 16, 2018)
- Brightfield and Fluorescence Imaging using 3D PrimeSurface? Ultra-Low Attachment Microplates (December 21, 2017)
- An Image-Based Method to Detect and Quantify T Cell Mediated Cytotoxicity of 2D and 3D Target Cell Models (December 13, 2017)
- Cost-Effective Automated Hepatotoxicity Testing using in vitro 3D Bioprinting (August 17, 2017)
- Automated Label-Free Cell Migration Assays using Magnetic 3D Bioprinting (August 16, 2017)
- Long-Term Hepatotoxicity Studies using Cultured Human iPSC-Derived Hepatocytes (August 15, 2017)
- Validation of an Image-Based 3D Natural Killer Cell Mediated Cytotoxicity Assay (March 24, 2017)
- An Automated Walk-Away System to Perform Differentiation of 3D Mesenchymal Stem Cell Spheroids (December 16, 2016)
- Automation and Image-Based Analysis of a Hanging Drop Micro-Hole Plate Model to Create and Differentiate 3D Mesenchymal Stem Cell Spheroids for Downstream Tissue Formation (October 25, 2016)
- Automated Monitoring of Protein Expression and Metastatic Cell Migration using 3D Bioprinted Colorectal Cancer Cells (September 23, 2016)
- Utilizing Automated Imaging and Advanced 3D Cell Culture Techniques to Quantify Apoptosis Activity (April 5, 2016)
- Image-Based Analysis of a Human Neurosphere Stem Cell Model for the Evaluation of Potential Neurotoxicants (March 25, 2016)
- Validation of High-Throughput Wound Healing Assay using 3D Cell Patterning and Automated, Kinetic Imaging (February 8, 2016)
- Z-Stacking and Z-Projection using a Scaffold-based 3D Cell Culture (May 7, 2015)
- Validation of a Novel Tumoroid-Based Cell Culture Model to Perform 3D in vitro Cell Signaling Analyses - Comparison of Results Generated with an in vitro Cell Signaling Assay using Cells Cultured in 2D and a Novel 3D Cell Culture System (April 30, 2015)
- Automation of HTRF? elF4E Kinase Assay Using a 3D Tumoroid- Based Cell Model (April 30, 2015)
- Combining Kinetic Ligand Binding and 3D Tumor Invasion Technologies to Assess Drug Residence Time and Anti-metastatic Effects of CXCR4 Inhibitors (April 16, 2015)
- Quantification of MMP Activity and Inhibition in a 3D Tumor Invasion Model (April 7, 2015)
- Z-Stacking of Single Plane Digital Widefield Fluorescent Images (October 31, 2014)
- Inhibition of Hypoxic Tumor Cells using a Three-Dimensional Spheroid Model (August 6, 2014)
- Live Cell Hypoxia Determination in a 3D Spheroid Model (August 5, 2014)
- Automated Cell Dispensing and Image-Based Spheroid Formation Tracking (April 15, 2014)
- The Effect of Cell Culture Method on Long-Term Primary Hepatocyte Cell Health (February 19, 2014)
ADME/Tox (20)
In-vitro assays using either cells or purified enzyme for Lead Optimization of small molecules in drug discovery; drug absorption, drug metabolism and drug cytotoxicity assays
- Automated Image Screening of Zebrafish Embryos Exposed to Developmental Toxins (January 4, 2019)
- Comparison of Oridonin Cytotoxicity in U-2 OS and HepG2 Cells (July 6, 2016)
- Oridonin Perfusion Causes Cytotoxicity in U-2 OS Cells (June 29, 2016)
- Detection of Human IgG Using a Luminescence-based Proximity Assay (December 5, 2014)
- The Impact of a 3-Dimensional Human Liver Microtissue Model on Long-term Hepatotoxicity Studies (March 31, 2014)
- Incorporation of a Novel 3D Cell Culture System to Perform in vitro Cytotoxicity Analyses using Human Primary Hepatocytes (March 20, 2014)
- Analysis of a New High Throughput Screening Detection Technology for Rapid hERG Safety Testing using a Fluorescence Polarization Assay (February 12, 2013)
- Automated 384-Well Cell-Based Cytochrome P450 Inhibition Assays Using Cryopreserved Human Hepatocytes in Suspension (January 16, 2012)
- A Simple, Robust Automated Multiplex Assay for Mitochondria Toxicity Testing (December 19, 2011)
- Overcoming the Crabtree Effect is Critical to Mitochondrial Toxicity Testing (October 24, 2011)
- Low Cost Solution for PAMPA Assays - Using the Epoch? Microplate Spectrophotometer with pION's PAMPA Explorer? for the In-vitro Drug Permeability Studies (June 16, 2011)
- A Fluorescent Microplate-Based Assay Workflow Enabling the Functional Characterization of Multi-Drug Resistance Transporters in Living Cells - Characterization of MDR Antiporter Expression in Tissue Culture Cell Lines (June 3, 2011)
- Automated Detection of Drug-Induced Lysosomal Cytotoxicity - Automation of the Lyso-ID? Red Assay Using the EL406? Combination Washer Dispenser (May 21, 2010)
- Fluorescent Detection of Drug-Induced Mitochondrial Toxicity (May 12, 2010)
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Automated Drug-Induced Chromatin Condensation Detection
Detection of End-Stage Apoptosis Using the Nuclear-ID? Kit from Enzo Life Sciences (February 17, 2010) - Quantifying Cytotoxicity of Thiostrepton on Mesothelioma Cells using MTT Assay and the Epoch? Microplate Spectrophotometer (December 10, 2009)
- Automation of a Multiplexed Cell-based Assay to Measure Simultaneously Induction of Gene Expression and Activity of Cytochrome P450 Isoform 3A4 by Small Molecule Compounds (December 10, 2009)
- Automated ApoTox-Glo Assay to Assess Cell Viability, Cytotoxicity and Apoptosis (March 31, 2009)
- An Absorbance-based Cytotoxicity Assay using High Absorptivity, Water-soluble Tetrazolium Salts - Cell Quantitation Using WST-8 and the Synergy? Mx (March 20, 2009)
- Invitrogen's Predictor? hERG Fluorescence Polarization Assay Using BioTek's Synergy? 4 Hybrid Microplate Reader - Non-Radioactive High Throughput Screening Assay for hERG Binding (July 21, 2008)
Automation (24)
Demonstration of automated procedures for various assays using microplates.
- Automated ELISA Liquid Handling with the EL406 Washer Dispenser (November 16, 2020)
- Automating the Wash Steps of ELISpot Assays using BioTek’s 405 TS Microplate Washer (March 18, 2020)
- Stimulation of Human Peripheral Blood Mononuclear Cells (March 10, 2020)
- Automated Kinetic Imaging Assay of Cell Proliferation in 384-Well Format (December 26, 2019)
- A High-Throughput Luminescence-Based Live Cell Assay to Measure ?-Arrestin Recruitment in Real Time (April 4, 2019)
- Incorporation of a Novel, Automated Scratch Tool and Kinetic Label-Free Imaging to Perform Wound Healing Assays (October 1, 2018)
- Automated Media Exchange for Spheroid Cultures Using a Novel MultiFlo? FX Accessory (July 31, 2018)
- Cost-Effective Automated Hepatotoxicity Testing using in vitro 3D Bioprinting (August 17, 2017)
- An Automated Walk-Away System to Perform Differentiation of 3D Mesenchymal Stem Cell Spheroids (December 16, 2016)
- Automation and Image-Based Analysis of a Hanging Drop Micro-Hole Plate Model to Create and Differentiate 3D Mesenchymal Stem Cell Spheroids for Downstream Tissue Formation (October 25, 2016)
- Applied Robotics for Enhanced Throughput Options in Microscopy As Demonstrated by Automated Tissue Microarray (TMA) Imaging (November 6, 2014)
- Efficacy of Using a Combination Microplate Washer for Vacuum-Based DNA Sequencing Reaction Clean-up (October 27, 2011)
- Automated Washing of Multiplex Bead Based Assays for the Luminex MAGPIX? Reader System - Comparison of Luminex 100? and MAGPIX? Luminex Readers (July 26, 2011)
- Multiplexing Magnetic Bead-based Human Cytokine Assays - Automated Magnetic Bead Washing Using the ELx405 (July 13, 2010)
- Automated Cell Dispensing into 1536-Well Microplates for HTS - Using the MicroFlo? Select to Seed Tissue Culture Cells (February 18, 2010)
- Versatile, Cost-Effective Automation of Avian Influenza and Mycoplasma Gallispeticum-Synovaie ELISAs (January 28, 2010)
- Automated Workflows for Luminex xMAP? Assays (November 6, 2009)
- Automated Magnetic Bead Washing of Metabolic Hormone Assays - Using the ELx405? Magnetic Bead Washer to Wash MILLIPLEX? MAP Assays (September 22, 2009)
- Automated Washing of Magnetic Bead QuantiGene? Plex 2.0 Assays - Using the ELx405? Magnetic Bead Washer to Wash Multiplexed mRNA Quantitation Assays (September 18, 2009)
- Automated ApoTox-Glo Assay to Assess Cell Viability, Cytotoxicity and Apoptosis (March 31, 2009)
- Using EL406? Combination Washer Dispenser to Semi-automate a Competitive ELISA for Melamine - Semi-Automated Melamine Quantitation (January 16, 2009)
- Aliquoting Agar Containing Solutions with the MicroFlo? Select Dispenser (April 28, 2008)
- Luminex xMAP Assays: Using BioTek’s ELx50? Filter Microplate Washer to Perform the Wash Processes Required (April 16, 2007)
- Automating Routine Microplate Pipetting and Dispensing (September 24, 2001)
Biochemical Assays (16)
Assays using purified drug target in Hit ID and Hit to Lead small molecule screening efforts.
- Receptor-Mediated Stimulation of AKTpan-kinase in NIH3T3 Cells (October 8, 2020)
- Rapid Measurement of IgG Using Fluorescence Polarization (September 15, 2017)
- Monitoring of Protein Kinase Activity Using Next Generation CSox-based Substrate Sensors (August 2, 2017)
- Comparative Analysis of Dual-Luciferase? Assay Technologies in a High Throughput Microplate Format (March 18, 2016)
- Automation of a HTRF? Universal Methyltransferase Assay (December 23, 2014)
- Analysis of α-Synuclein Fibril Formation in vitro (December 10, 2014)
- Detection of Human IgG Using a Luminescence-based Proximity Assay (December 5, 2014)
- Utility of Synergy? H4 Hybrid Multi-Mode Microplate Reader using both Monochromator- and Filter-Based Detection - Capabilities demonstrated with Fluorescence Polarization, Time-Resolved Fluorescence Resonance Energy Transfer, and Fluorescence Intensity Assays for the Measurement of ADP Accumulation (February 24, 2010)
- Optimization and Validation of Millipore’s HTRF? PI3 Kinase Assay for Inhibitors to Class IA Phosphionositide 3-Kinase Isoform Activity (July 10, 2009)
- LanthaScreen? TR-FRET Assay from Invitrogen on Synergy 4 (October 10, 2008)
- Gyrasol Kinase Assays (September 26, 2008)
- Optimizing Performance of the Transcreener? ADP Assay for the BioTek Synergy 2 and Synergy 4 Multi-Mode Microplate Readers (September 17, 2008)
- Invitrogen’s Adapta? Universal Kinase Assay using BioTek’s Synergy? 4 Hybrid Microplate Reader (May 20, 2008)
- Omnia Kinase Assay from Invitrogen on Synergy 4 (December 19, 2007)
- Z’-LYTE? Kinase Assay from Invitrogen? on Synergy? 4 Multi-Mode Microplate Reader (November 15, 2007)
- Mycoplasma Detection Using Synergy? 2 Multi-Mode Microplate Reader and the Lonza MycoAlert Assay (December 8, 2006)
Biofuel Research (7)
Assays assisting in R&D efforts to produce biofuels from corn, lignocellulosic material and algae.
- Chemical and Biochemical Means to Detect Alcohol - Determination of Ethanol Concentration in Fermented Beer Samples and Distilled Products (May 29, 2012)
- Enzymatic Digestion of Polysaccharides (Part I) (February 27, 2012)
- Enzymatic Digestion of Polysaccharides (Part II) (February 27, 2012)
- Monitoring Enzymatic Glucose Production from Cellulosic Feedstock (December 19, 2011)
- Determination of Algal Cell Lipids Using Nile Red - Using Microplates to Monitor Neutral Lipids in Chlorella Vulgaris (July 12, 2011)
- Monitoring of Algal Growth Using Their Intrinsic Properties (July 11, 2011)
- Identification of Biofuel Producing Bacteria Through Temperature Resistance - Demonstration of the High Temperature Capabilities of BioTek’s Microplate Readers (March 5, 2009)
Biomarkers (14)
Quantification of biological markers (i.e. proteins, DNA, RNA, etc.) associated with disease, toxicity and drug efficacy from biofluids (i.e. serum/plasma).
- Stimulation of Human Peripheral Blood Mononuclear Cells (March 10, 2020)
- Stimulation of IL-2 Secretion in Human Lymphocytes (March 21, 2018)
- Analysis of the Effect of Aggregated β-Amyloid on Cellular Signaling Pathways Critical for Memory in Alzheimer’s Disease (March 21, 2016)
- High Throughput, Multiplexed Detection of Inflammatory Cytokines in an Astrocyte and Monocyte Co-culture Model (February 18, 2016)
- Multiplexed Detection of Cytokine Cancer Biomarkers using Fluorescence RNA In Situ Hybridization and Cellular Imaging (February 18, 2016)
- Determination of IL-6 Cytokine levels by ELISA with Resonance Raman Signal Based Peroxidase Detection (August 14, 2014)
- Automation of Optimiser? Microplate Technology for High Throughput Applications (March 13, 2013)
- Differential Cancer Biomarker Secretion of Cultured Cells - Use of the ELx50? to Automate the Wash Steps of Multiplexed Luminex? xMAP? Bead Assays (July 26, 2011)
- Amplifying Immunoassay Sensitivity with the Optimiser? Microplate Technology - Repetitive Sample Loading of the Optimiser Microplate Allows for Amplification of Assay Sensitivity from Picogram/mL Levels to Femtogram/mL Levels (June 15, 2011)
- Determination of Insulin Levels in Human Serum - Using the ELx405? Microplate Washer and the Synergy? Mx Monochromator- Based Multi-Mode Microplate Reader to Perform an Insulin ELISA (July 1, 2009)
- Quantitation of Human TNF-a with a QuantiGlo Assay from R&D Systems Using BioTek’s Synergy? 2 Multi-Mode Microplate Reader - Luminescent ELISA Measurements (May 21, 2008)
- AlphaLISA: Human Insulin Quantification Using Synergy 4 Hybrid Multi-Mode Microplate Reader (December 10, 2007)
- cAMP and Tumor Necrosis Factor Quantification Using Synergy 2 Multi-Mode Microplate Reader and HTRF? Assays for High-Throughput Screening (March 26, 2007)
- Cholesterol Quantification Using a Fluorometric Assay and Synergy HT Multi-Mode Microplate Reader (February 28, 2005)
Biotherapeutics (11)
Microplate assays used for biologic drug discovery and development: clonal selection, characterization, formulation studies and immunogenicity.
- Comparison of Assay Methods for the Detection of Residual Protein A in Biological Therapeutics (July 14, 2015)
- Automation of a Homogeneous Proximity Assay for Detection of Residual Protein A in Biological Therapeutics (November 14, 2014)
- Automation of a Homogeneous Proximity Assay for Immunogenicity Testing of Biological Drug Products (June 5, 2013)
- An Automated DELFIA ADCC Assay Method using a CD16.NK-92 Cell Line (March 7, 2013)
- Automated Bioluminescent ADCC Reporter Bioassay Using Bioengineered Jurkat Cells (February 8, 2013)
- Automated High Throughput Functional Cell-based Bioassays for EGFR Blocking Antibodies (June 7, 2012)
- A Semi-Automated, Non-Radioactive Assay for the Detection of Antibody-Based Complement-Dependent Cytotoxicity (March 26, 2012)
- Automated Cellular Screening and Characterization of Therapeutic Antibodies for Antibody-Dependent Cell- Mediated Cytotoxicity Utility (February 3, 2012)
- Semi-Automation of a Non-Radioactive Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) Assay (Part I) (January 31, 2012)
- Semi-Automation of a Non-Radioactive Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) Assay (Part II) (January 31, 2012)
- A Novel Protein Aggregation Assay for Biologics Formulation Studies and Production QA/QC - ProteoStat? Fluorescent Reagent for Microplate-based Aggregate Quantitation (March 18, 2011)
Cell-based Assays (46)
Quantitative assays of cellular function using a wide range of microplate reader optical detection modes.
- Receptor-Mediated Stimulation of AKTpan-kinase in NIH3T3 Cells (October 8, 2020)
- Automated Colony Formation Assay (May 1, 2020)
- A Homogeneous FRET-based HTS Assay for Quantification of pRb in Cancer Cell Lines to Monitor Inhibition of G0/ G1 Cell Phase Transition (April 21, 2020)
- Multi-Mode Metabolic Profiling of Stationary Phase Saccharomyces Cerevisiae Exposed to Short-Term Oxidative or Nutrient Stress (March 25, 2020)
- Stimulation of Human Peripheral Blood Mononuclear Cells (March 10, 2020)
- High-Throughput Fluorescent Colony Formation Assay (January 10, 2020)
- Automated Kinetic Imaging Assay of Cell Proliferation in 384-Well Format (December 26, 2019)
- Automated Label-Free Method for Measuring Cell Migration in Real-Time with Oris Pro Assay (May 21, 2019)
- A High-Throughput Luminescence-Based Live Cell Assay to Measure ?-Arrestin Recruitment in Real Time (April 4, 2019)
- Automated Image Screening of Zebrafish Embryos Exposed to Developmental Toxins (January 4, 2019)
- Incorporation of a Novel, Automated Scratch Tool and Kinetic Label-Free Imaging to Perform Wound Healing Assays (October 1, 2018)
- Investigation of Protein: Protein Interactions (PPI) using a Novel Bioluminescence Resonance Energy Transfer (BRET) Proximitybased, High Throughput Screening Assay (June 17, 2016)
- Analysis of the Effect of Aggregated β-Amyloid on Cellular Signaling Pathways Critical for Memory in Alzheimer’s Disease (March 21, 2016)
- High Throughput, Multiplexed Detection of Inflammatory Cytokines in an Astrocyte and Monocyte Co-culture Model (February 18, 2016)
- Automation of a Homogeneous Proximity Assay for Detection of ERK1/2 or SMAD3 Phosphorylation (July 14, 2015)
- Label-Free Phenotype MicroArrays? Analysis of Cellular Energetics and Apoptotic Activity using Microplate Reading and Phase Contrast Imaging (April 13, 2015)
- Live Cell Imaging of GPCR Dependent Second-Messenger Systems (April 14, 2014)
- Live Cell Biosensor Assay Used To Interrogate GPCRs - Comparative Analysis of Biosensor Variants (November 8, 2013)
- Validation and Comparison of Single-Step Flash and Dual-Spectral Luciferase Reporter Gene Assays using the Synergy? Line of Microplate Readers (October 16, 2013)
- Multiplexed Assay for IL-6 Secretion and Cell Viability Using an Epithelial Ovarian Cancer Cell Line (April 15, 2013)
- Automating a Direct, Cell-Based, Target-Compound Interaction Assay for G9a Histone Methyltransferase and Bromodomain Proteins (February 8, 2013)
- Automated, High Throughput Methods for the Quantification of Endogenous Cellular Kinase Activity using HTRF Technology (January 28, 2013)
- Determination of Endogenous Cellular Kinase Activity Through the Use of an Automated, High Throughput AlphaScreen?-Based Assay (September 13, 2012)
- Rapid Screening of a Cell-based Assay for GLP-1 Receptor Using a Natural Product Library (August 7, 2012)
- Automation of a Novel Cell-Based ELISA for Cell Signaling Pathway Analysis (May 16, 2012)
- An Automated, Cell-based Platform for the Rapid Detection of Novel Androgen Receptor Modulators (March 7, 2012)
- A Cost-effective Workflow for High-Throughput Screening of GProtein Coupled Receptors (GPCRs) - Monitoring Receptor Mediated Calcium Flux (June 3, 2011)
- A Homogeneous Assay to Quantify Endogenous AKT Phosphorylation in Human Umbilical Vein Endothelial Cells (February 22, 2011)
- HTRF? Ligand Binding Assay for the Chemokine Receptor CXCR4 (October 29, 2010)
- Automation of a Bioluminescent Live-Cell cAMP Assay for the Pharmacological Evaluation of GPCRs (October 29, 2010)
- Live-Cell Assay to Interrogate GPCRs by Monitoring cAMP Levels using a Bioluminescent Readout (October 19, 2010)
- Cell-based Assay for the Quantification of mTORC1 Activation and Inhibition in a Breast Cancer Cell Line - Endogenous Cell-based Kinase Assay using the AlphaScreen? SureFire? p70 S6 Kinase Kit and Synergy Alpha (July 14, 2010)
- Optimization of Perkin Elmer’s AlphaScreen? cAMP Assay on the Synergy? H4 Hybrid Multi-Mode Microplate Reader (March 18, 2010)
- Automated Processing of a Homogeneous Phospho-ERK1/2 Assay for Use as a Generic GPCR Sensor - Homogeneous LanthaScreen? Cellular Assay (February 1, 2010)
- BacMam-Enabled Cellular Assays Measuring Histone Posttranslational Modifications (December 22, 2009)
- Cell-Based Assay Preparation in 1536-well Microplates using BioTek’s EL406? Microplate Washer Dispenser (July 1, 2009)
- HTRF? cAMP/IP-One HTplex? Cell-Based Assay performed on BioTek’s Synergy? 2 Multi-Mode Microplate Reader (June 17, 2009)
- Optimizing Performance of the Membrane-free, Oris? Cell Migration Assay for High Throughput Screening using the BioTek Synergy? HT Multi-Mode Microplate Reader (April 9, 2009)
- Using BioTek's Synergy HT Reader to Measure Reactive Oxygen Species (ROS) Generation in Stimulated Cells (November 5, 2008)
- Oris - A Novel High Throughput-Compatible Cell Migration Screening Assay (August 21, 2008)
- GeneBLAzer? FRET Cell-based Assay from Invitrogen? on Synergy? 4 Hybrid Multi-Mode Microplate Reader (December 20, 2007)
- AlphaScreen?: cAMP Quantification Using Synergy 2 Multi-Mode Microplate Reader (December 10, 2007)
- FRET-Based Ion Channel, GPCR, and Kinase Assay Applications on the Synergy? HT Multi-Detection Microplate Reader from BioTek (March 1, 2005)
- Optimizing Dual-Glo? Luciferase Assays with Synergy? HT Multi-Mode Microplate Reader (February 23, 2005)
- Using Synergy? HT Multi-Mode Microplate Reader to Run the Dual-Luciferase? Reporter Assay System (February 15, 2005)
- Ion Channel Assay Development on the Synergy? HT Multi Detection Microplate Reader from BioTek (January 29, 2004)
Cellular Imaging (36)
Imaging of cells after fixation or lysis using fluorescence microscopy.
- Image Analysis of Herpesvirales Specificity Challenge using Direct Fluorescence Antibody Assay (DFA) (December 10, 2020)
- Detection and Automated Imaging of Regions of Interest (ROIs) when Performing Whole Slide Imaging (WSI) (May 15, 2020)
- Automated Colony Formation Assay (May 1, 2020)
- Multi-Mode Metabolic Profiling of Stationary Phase Saccharomyces Cerevisiae Exposed to Short-Term Oxidative or Nutrient Stress (March 25, 2020)
- Stimulation of Human Peripheral Blood Mononuclear Cells (March 10, 2020)
- High-Throughput Fluorescent Colony Formation Assay (January 10, 2020)
- Automated Scoring of Acid-fast Bacteria (AFB) by Fluorescent Smear Microscopy (October 16, 2019)
- Nuclear Translocation of RelA in Stimulated Macrophages (September 18, 2019)
- Quantitative Image Analysis of Bacilli Viability in Agar Mounted Soil Samples (July 10, 2019)
- Automated Label-Free Method for Measuring Cell Migration in Real-Time with Oris Pro Assay (May 21, 2019)
- Automated Analysis of Neutrophil NETosis Activity (April 30, 2019)
- Analyzing Wound Healing in Zebrafish Embryos (February 11, 2019)
- Quantitative Microscopy of Angiogenesis in Zebrafish Embryos (December 6, 2018)
- Determination of Cell Cycle Time (December 6, 2018)
- Incorporation of a Novel, Automated Scratch Tool and Kinetic Label-Free Imaging to Perform Wound Healing Assays (October 1, 2018)
- XF Data Normalization by the Agilent Seahorse XF Imaging and Normalization System (September 5, 2018)
- Monitoring Cell Cycle Progression in Cancer Cells (July 3, 2018)
- Stimulation of IL-2 Secretion in Human Lymphocytes (March 21, 2018)
- Using Acridine Orange to Measure Cell Death in Ethanol Treated Zebrafish Embryos (December 27, 2017)
- Automated Imaging and Dual-Mask Spot Counting of γH2AX Foci to Determine DNA Damage on an Individual Cell Basis (December 19, 2017)
- Monitoring Cell Cycle Progression Using Microscopy (August 22, 2017)
- Lipid Accumulation in HepG2 Cells Exposed to Free Fatty Acids (August 14, 2017)
- Comparison of Different Cell Types for Neutral Lipid Accumulation (August 14, 2017)
- Automation of Non-Alcoholic Fatty Liver Disease Model Assay (August 14, 2017)
- Automated Monitoring of Protein Expression and Metastatic Cell Migration using 3D Bioprinted Colorectal Cancer Cells (September 23, 2016)
- Automated Comet Assay Imaging and Dual-Mask Analysis to Determine DNA Damage on an Individual Comet Basis (July 7, 2016)
- Analysis of the Effect of Aggregated β-Amyloid on Cellular Signaling Pathways Critical for Memory in Alzheimer’s Disease (March 21, 2016)
- Detection and Visual Localization of Individual Cellular Proteins Using a Proximity Ligation Assay (March 4, 2016)
- Multiplexed Detection of Cytokine Cancer Biomarkers using Fluorescence RNA In Situ Hybridization and Cellular Imaging (February 18, 2016)
- High Content RNAi Screening Using Printed Libraries (February 9, 2016)
- Analysis of Cell Retention Following Rigorous Automated Cell Fixation and Staining (December 18, 2015)
- Automated Imaging and Analysis of a Novel Comet Assay to Enable High Throughput Genotoxicity Testing (December 1, 2015)
- Phenotypic and Epigenetic Mechanism of Action Determinations of Histone Methylase and Demethylase Inhibitors using Digital Widefield Microscopy (April 10, 2015)
- High Resolution Fluorescence Microscopy of PtK2 Cells Undergoing Mitosis in Microplates (June 24, 2014)
- Analysis of Nuclear Stained Cells - Using the Cytation?3 Cell Imaging Multi-Mode Microplate Reader with DAPI-Stained Cells (April 15, 2013)
- Automated Tissue Culture Cell Fixation and Staining in Microplates (April 15, 2013)
Enzymology (11)
Kinetic studies using purified enzymes in a microplate format.
- Monitoring Protein Dehydrogenase Activity with Filter-based Absorbance Spectroscopy (September 22, 2017)
- Monitoring of Protein Kinase Activity Using Next Generation CSox-based Substrate Sensors (August 2, 2017)
- Determining NADH Concentrations with Synergy? 2 Multi-Mode Microplate Reader using Fluorescence or Absorbance (November 26, 2007)
- Kinetic Analysis of ?-Galactosidase Activity using PowerWave? HT Microplate Spectrophotometer and Gen5? Data Analysis Software (February 26, 2007)
- Caspase Activity Using Synergy HT Multi-Mode Microplate Reader: Measuring Apoptosis Enzymes (July 28, 2004)
- Horseradish Peroxidase (HRP) Determination Using Amplex Red and Synergy HT Multi-Mode Microplate Reader (April 21, 2003)
- Hydrogen Peroxide Quantification Using Synergy HT Multi-Mode Microplate Reader (August 20, 2002)
- ATPase Activity Determination Using BioTek's ELx808 Microplate Reader (September 24, 2001)
- ?-Galactosidase Activity Determination using a BioTek Microplate Fluorescence Reader (February 20, 2001)
- BioTek PowerWave 200 Microplate Spectrophotometer: Nitric Oxide Synthesis (February 7, 2001)
- Phosphate and ATPase Determination Using the μQuant Scanning Microplate Spectrophotometer (February 6, 2001)
Epigenetics (10)
Cell-based and biochemical assays to measure activity and small molecule modulation of epigenetic target proteins.
- Phenotypic and Epigenetic Mechanism of Action Determinations of Histone Methylase and Demethylase Inhibitors using Digital Widefield Microscopy (April 10, 2015)
- Automation of a HTRF? Universal Methyltransferase Assay (December 23, 2014)
- Assessment of HDAC1 Inhibition Using an Automated, Bioluminescent Histone Deacetylase I/II Assay (March 27, 2013)
- Automated, High Throughput, HTRF?-Based Detection of Histone Methyltransferase and Demethylase Enzyme Activity (March 5, 2013)
- Interrogation of SET7/9 Histone Lysine Methyltransferase Using a High Throughput Fluorescence Polarization Screening Assay (February 12, 2013)
- BRD4 Bromodomain 1 Inhibitor Screening using a Homogeneous Proximity Assay (February 11, 2013)
- Automating a Direct, Cell-Based, Target-Compound Interaction Assay for G9a Histone Methyltransferase and Bromodomain Proteins (February 8, 2013)
- Automation of a Generic Fluorescent Methyltransferase Activity Assay (March 9, 2012)
- A Flexible and Robust Solution for Automated 384-well HDAC Profiling (July 26, 2011)
- BacMam-Enabled Cellular Assays Measuring Histone Posttranslational Modifications (December 22, 2009)
Food Safety & Quality (7)
Assays measuring contaminants in food and beverages including bacteria and processing by-products that influence customer health and product quality.
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A Novel Food Freshness Assay Based on ATP Depletion
Part 2: Challenge Testing of the Automated Assay (August 13, 2014) -
A Novel Food Freshness Assay Based on ATP Depletion
Part 1: Automation and Sample Prep Optimization (August 8, 2014) - Concentration and Detection of Low Levels of Escherichia coli O157:H7, Listeria monocytogenes 4b, and Salmonella enterica Typhimurium in High Organic Load Lettuce Wash (March 6, 2014)
- Determination of Antioxidant potential using an Oxygen Radical Absorbance Capacity (ORAC) Assay with Synergy? H4 (August 7, 2012)
- Chemical and Biochemical Means to Detect Alcohol - Determination of Ethanol Concentration in Fermented Beer Samples and Distilled Products (May 29, 2012)
- Using EL406? Combination Washer Dispenser to Semi-automate a Competitive ELISA for Melamine - Semi-Automated Melamine Quantitation (January 16, 2009)
- Histamine Analysis in Wine Samples Using the Microplate Format (August 29, 2006)
Histology (3)
The study of the microscopic anatomy of tissues by sectioning and staining, typically with colored dyes such as H&E using an optical microscope.
Live Cell Imaging (39)
Kinetic imaging of live cells under environment control using brightfield, high contrast brightfield. phase contrast and fluorescence microscopies.
- Monitoring pH in Long-term Proliferation Assays (August 20, 2020)
- Multi-Mode Metabolic Profiling of Stationary Phase Saccharomyces Cerevisiae Exposed to Short-Term Oxidative or Nutrient Stress (March 25, 2020)
- Automated Kinetic Imaging Assay of Cell Proliferation in 384-Well Format (December 26, 2019)
- Nuclear Translocation of RelA in Stimulated Macrophages (September 18, 2019)
- Automated Label-Free Method for Measuring Cell Migration in Real-Time with Oris Pro Assay (May 21, 2019)
- Automated Analysis of Neutrophil NETosis Activity (April 30, 2019)
- α-SMA Expression in Hepatic Stellate Cells (December 21, 2018)
- Quantitative Microscopy of Angiogenesis in Zebrafish Embryos (December 6, 2018)
- Advanced Wound Healing Assay Workflow using Automated Scratch Wound Creation, High Contrast Brightfield and Fluorescence Kinetic Imaging (December 6, 2018)
- Thapsigargin-induced Cellular Stress Response and Inhibition of Gq-dependent Calcium Signaling (October 5, 2018)
- Incorporation of a Novel, Automated Scratch Tool and Kinetic Label-Free Imaging to Perform Wound Healing Assays (October 1, 2018)
- 3D Spheroid-Based Tumor Invasion Assay (July 16, 2018)
- Characterizing Gs- and Gi-dependent Regulation of cAMP Levels in Real Time with cADDis Biosensor and Automated Live Cell Imaging (March 29, 2018)
- Live Cell Imaging of Apoptosis and Necrosis (March 23, 2018)
- Using Phenol Red to Assess pH in Tissue Culture Media (March 22, 2018)
- Automated, Multi-Parameter, Kinetic Methods to Quantify Cell Death (March 22, 2018)
- Image-Based, Label-Free Methods to Detect and Quantify General and Directed T Cell Activation (December 8, 2017)
- Automated Imaging Assay for Characterizing Ca2+ Flux with R-GECO Biosensor (September 28, 2017)
- Mitochondrial Membrane Potential by Object Spot Counting (September 11, 2017)
- Monitoring Cell Cycle Progression Using Microscopy (August 22, 2017)
- Autophagy Analysis Using Object Spot Counting (July 11, 2017)
- Normalization of Agilent Seahorse XF Data by In-situ Cell Counting Using a BioTek Cytation 5 (May 16, 2017)
- Characterizing Calcium Mobilization using Kinetic Live Cell Imaging (January 26, 2017)
- Kinetic Proliferation Assay Using Label-Free Cell Counting (January 25, 2017)
- Monitoring Saccharomyces cerevisiea Growth with Brightfield Microscopy in Real Time (July 20, 2016)
- Monitoring Viral Infection of Mammalian Cells using Digital Fluorescence Microscopy (July 5, 2016)
- Performance of a Label-Free Image-Based 2D Scratch Wound Healing Assay to monitor Cell Migration and its Inhibition (June 29, 2016)
- Monitoring Cytotoxicity by Oridonin in U-2 OS Cells in Real Time (May 20, 2016)
- Label-Free Phenotype MicroArrays? Analysis of Cellular Energetics and Apoptotic Activity using Microplate Reading and Phase Contrast Imaging (April 13, 2015)
- Imaging and Analysis of a High Density Cell Migration Assay (November 25, 2014)
- Automated Hemocytometer-Based Live/Dead Cell Counting using Phase Contrast and Color Brightfield Imaging (November 4, 2014)
- Use of Phase Contrast Imaging to Track Morphological Cellular Changes due to Apoptotic Activity (October 31, 2014)
- Cell Synchrony Determination Using Microscopic Imaging - Use of Nuclear Staining to Assess Cell Cycle Stage by Nuclear DNA Content (October 14, 2014)
- Automated, Kinetic Imaging of Cell Migration and Invasion Assays using Corning FluoroBlok? Permeable Supports (October 9, 2014)
- Live Cell Imaging of RNA Expression (April 18, 2014)
- Live Cell Imaging of GPCR Dependent Second-Messenger Systems (April 14, 2014)
- Investigation of Cell Migration using a High Density Cell Exclusion Assay and Automated Microplate Imager (July 10, 2013)
- Induction and Inhibition Studies of Hypoxia and Oxidative Stress in Immortalized Keratinocytes (June 13, 2013)
- Imaging of BacMam Transfected U-2 OS Cells (April 15, 2013)
Nucleic Acid Quantification (18)
Quantification of dsDNA, ssDNA, RNA and µRNA using micro-volume, microplate and 1 cm pathlength devices for downstream processes such as PCR, qPCR and sequencing.
- Quantification of dsDNA using Qubit? dsDNA Broad Range (BR) Assay Kit (December 6, 2018)
- Fluorometric Quantitation of dsDNA using PicoGreen? (January 25, 2017)
- Low-volume, High Throughput Workflow for Analysis of Nucleic Acid Samples for Biobanking (December 18, 2015)
- Micro-Volume Purity Assessment of Nucleic Acids using A260/A280 Ratio and Spectral Scanning (June 5, 2012)
- Analytical Performance of a Higher Throughput Micro-Volume Microplate Accessory for Microplate Spectrophotometer Platforms (November 10, 2011)
- Micro-Volume PicoGreen? DNA Quantification using BioTek’s Take3? Multi-Volume Plate (June 2, 2010)
- Analytical Performance of Nucleic Acid Micro-Volume Quantification Using the Epoch? Spectrophotometer System (December 9, 2009)
- Compatibility of Synergy? Multi-Mode Microplate Readers for Micro-Volume Nucleic Acid Quantification (November 9, 2009)
- Multi-Volume Analysis of Nucleic Acids Using the Epoch? Spectrophotometer System (November 3, 2009)
- Low Volume Nucleic Acid Quantification using a Multi-Volume Spectrophotometer System (August 17, 2009)
- RNA Quantification with RiboGreen Stain Using the FL600 Fluorescence Microplate Reader (September 5, 2003)
- DNA Quantification using Hoechst 33258 (February 21, 2001)
- ssDNA Quantification Using OliGreen Fluorescent Stain (February 21, 2001)
- Nucleic Acid & Protein Quantitation in the Microplate Format (February 21, 2001)
- Fluorometric Hydrolyzed DNA Quantification (February 20, 2001)
- Increasing the DNA Quantification Range when using Hoechst Dye 33258 (February 9, 2001)
- BioTek PowerWave HT Microplate Spectrophotometer: Quantitation of Nucleic Acids (February 6, 2001)
- Nucleic Acid Purity Assessment Using A260/A280 Ratios (February 6, 2001)
Physical Sciences (3)
Determination of fundamental constants of physical chemistry.
- Utility of Variable Bandwidth Monochromators for Quantification of Fluorescent Probes in Produced Effluent Water (July 1, 2015)
- Rapid Critical Micelle Concentration (CMC) Determination Using Fluorescence Polarization (September 18, 2014)
- Determination of Fluorescence Excitation and Emission Peaks for Qdot? Nanocrystals Using the Synergy? 4 Multi-Mode Microplate Reader - Using Monochromators for Fluorescence (June 28, 2007)
Protein Quantification (18)
Quantification of total protein using micro-volume, microplate and 1 cm pathlength devices for downstream processes such as PAGE and mass spectrometry.
- Receptor-Mediated Stimulation of AKTpan-kinase in NIH3T3 Cells (October 8, 2020)
- Rapid Measurement of IgG Using Fluorescence Polarization (September 15, 2017)
- Protein Quantitation using NanoOrange? Fluorescence (January 26, 2017)
- Automation of a Homogeneous Proximity Assay for Detection of Residual Protein A in Biological Therapeutics (November 14, 2014)
- UV Fluorescence Polarization as a Means to Investigate Protein Conformational and Mass Change - Using Intrinsic Tryptophan Fluorescence in Conjunction with UV-capable Polarizers (October 13, 2014)
- Micro-Volume Determination of Labeling Efficiency Using Spectral Scanning Analysis in a Standard Microplate Reader (February 11, 2013)
- Micro-Volume Purity Assessment of Nucleic Acids using A260/A280 Ratio and Spectral Scanning (June 5, 2012)
- Analytical Performance of a Higher Throughput Micro-Volume Microplate Accessory for Microplate Spectrophotometer Platforms (November 10, 2011)
- In-situ Micro-Volume Bicinchoninic Acid Protein Assay (February 26, 2010)
- Analytical Performance of Epoch? Multi-Volume Spectrophotometer System for Protein Quantification (January 21, 2010)
- Peptide and Amino Acid Quantification Using UV Fluorescence in Synergy HT Multi-Mode Microplate Reader (April 18, 2003)
- Nucleic Acid & Protein Quantitation in the Microplate Format (February 21, 2001)
- Using a BioTek Fluorescence Microplate Reader for Chemiluminescence Detection (February 21, 2001)
- Total Protein Determination By the Lowry Method Using the ELx808 (February 20, 2001)
- Fluorescence-based Total Protein Determination using the Compound CBQCA (February 20, 2001)
- Fluorimetric Protein Quantification Using the Reactive Compound Fluorescamine (February 20, 2001)
- Green Fluorescent Protein Quantification in Microplates (February 20, 2001)
- Total Protein Quantification using OPA (February 20, 2001)
Stem Cell Differentiation (2)
Assay workflows that monitor and validate stem cell differentiation.
- An Automated Walk-Away System to Perform Differentiation of 3D Mesenchymal Stem Cell Spheroids (December 16, 2016)
- Automation and Image-Based Analysis of a Hanging Drop Micro-Hole Plate Model to Create and Differentiate 3D Mesenchymal Stem Cell Spheroids for Downstream Tissue Formation (October 25, 2016)
Suspension Cell Growth (4)
Spectrophotometric measurements of bacteria, yeast and other non-adherent cells based on light scatter, typically at A600.
- Monitoring Bacterial Growth under Different Environmental Conditions (July 16, 2020)
- Monitoring Cell Growth in 2 μL Volumes Using a Microplate Reader (December 19, 2011)
- Monitoring Growth of Beer Brewing Strains of Saccharomyces Cerevisiae - The Utility of Synergy H1 for Providing High Quality Kinetic Data for Yeast Growth Applications (November 17, 2010)
- Monitoring the Growth of E. coli With Light Scattering Using the Synergy? 4 Multi-Mode Microplate Reader with Hybrid Technology? (January 15, 2008)
Veterinary Analysis (3)
Assays used in animal husbandry to assess health and disease.
- Fluorescence Polarization Detection of Brucella abortus and Mycobacterium bovis specific antibodies Using Kits from Diachemix - Veterinary Diagnosis of Brucellosis and Tuberculosis (July 21, 2010)
- Versatile, Cost-Effective Automation of Avian Influenza and Mycoplasma Gallispeticum-Synovaie ELISAs (January 28, 2010)
- Automating the IDEXX Avian Influenza Virus Antibody Test Kit with Microplate Instrumentation and BioTek Twister II (March 4, 2008)